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p3 primary cell nucleofector buffer  (Lonza)


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    Lonza p3 primary cell nucleofector buffer
    P3 Primary Cell Nucleofector Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p3+primary+buffer/4d+nucleofector/pm40570854-740-8-7
    Average 90 stars, based on 1 article reviews
    p3 primary cell nucleofector buffer - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Generation of gene-corrected isogenic control cell lines from a DYT1 dystonia patient iPSC line carrying a heterozygous GAG mutation in TOR1A gene
    Article Snippet: Briefly, approximately 1 × 10 6 single cells were resuspended in P3 primary buffer (Lonza) with gRNA/Cas9 ribonuclease protein (RNP) complex (200 pmol synthetic gRNA and 80 pmol HiFi Cas9 protein) and TOR1A correction ssODN ( ).

    Article Title: Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation P525L (c.1574C > T) in FUS gene.
    Article Snippet: Mutations in the FUS (fused in sarcoma) gene are implicated in the neurodegenerative disease amyotrophic lateral sclerosis (ALS).. However, the pathophysiology underlying these mutations remains elusive.. In this study, we created two induced pluripotent stem cell (iPSC) lines through genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A).

    Article Title: Generation of gene-corrected isogenic control cell lines from a DYT1 dystonia patient iPSC line carrying a heterozygous GAG mutation in TOR1A gene.
    Article Snippet: Briefly, approximately 1 × 106 single cells were resuspended in P3 primary buffer (Lonza) with gRNA/Cas9 ribonuclease protein (RNP) complex (200 pmol synthetic gRNA and 80 pmol HiFi Cas9 protein) and TOR1A correction ssODN (Table 2).

    Mutagenesis:

    Article Title: Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation P525L (c.1574C > T) in FUS gene
    Article Snippet: .. To begin, about 1 million single cells were suspended in P3 primary buffer (Lonza), along with a gRNA/Cas9 ribonuclease protein (RNP) complex consisting of 200 pmol synthetic gRNA, 80 pmol SpCas9 protein, and hFUS mutant ssODN ( ). .. The cells were then electroporated with a 4DNucleofector (Lonza) using the CA-137 program.

    Article Title: Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene
    Article Snippet: .. Briefly, approximately 1 × 10 6 single cells were resuspended in P3 primary buffer (Lonza) with gRNA/Cas9 ribo-nuclease protein (RNP) complex (200 pmol synthetic gRNA and 80 pmol SpCas9 protein) and hFUS mutant ssODN ( ). .. Subsequently, cells were electroporated with a 4DNucleofector (Lonza) using CA-137 program.

    Article Title: Generation of two induced pluripotent stem cell lines with heterozygous and homozygous amyotrophic lateral sclerosis-causing mutation R521G (c.1561C > G) in FUS gene.
    Article Snippet: Mutations in the RNA-binding protein FUS (fused in sarcoma) are linked to amyotrophic lateral sclerosis (ALS), but the pathogenesis is not fully understood.. For modeling ALS, here we generated two induced pluripotent stem cell (iPSC) lines carrying the heterozygous and homozygous R521G (c.1561C > G) mutation in the FUS gene via genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A).. Both lines show normal stem cell morphology and karyotype, express pluripotent markers, and can differentiate into three germ layers, providing a valuable resource in determining the pathological mechanisms underlying the FUS mutation of R521G in ALS.

    Suspension:

    Article Title: TIM-3, LAG-3, or 2B4 gene disruptions increase the anti-tumor response of engineered T cells.
    Article Snippet: .. Two million cells/condition were resuspended in P3 primary buffer (Lonza) and assembled RNPs targeting one or more coding sequences of selected genes were simultaneously added to the cell suspension. .. Cells were electroporated using Nucleofector 4D (Lonza) according to manufacturer’s instructions; 24 hours later, the electroporated cells were counted and transduced with a previously validated lentiviral vector encoding for an HLA-A2 restricted NY-ESO-1157-165 specific TCR.

    Article Title: TIM-3, LAG-3, or 2B4 gene disruptions increase the anti-tumor response of engineered T cells
    Article Snippet: .. Two million cells/condition were resuspended in P3 primary buffer (Lonza) and assembled RNPs targeting one or more coding sequences of selected genes were simultaneously added to the cell suspension. .. Cells were electroporated using Nucleofector 4D (Lonza) according to manufacturer’s instructions; 24 hours later, the electroporated cells were counted and transduced with a previously validated lentiviral vector encoding for an HLA-A2 restricted NY-ESO-1 157-165 specific TCR.



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